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CD4 抗体

This anti-CD4 antibody is a 小鼠单克隆 antibody detecting CD4 in FACS, IP 和 Func. Suitable for 人.

产品编号 ABIN120586

发货至: 中国

Quick Overview for CD4 抗体 (ABIN120586)

抗原

See all CD4 抗体

CD4

适用

399
249
133
48
30
24
20
18
17
11
11
11
11
10
10
10
9
9
9
5
4
2
1
1
1
1
1

人

宿主

416
155
152
7
3
3
1
1

小鼠

克隆类型

单克隆

标记

This CD4 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

克隆位点

MEM-115

Discover our top product CD4 Primary Antibody

特异性

The antibody MEM-115 recognizes an epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (helper T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.

纯化方法

Protein A affinity chromatography

纯度

> 95 % (by SDS-PAGE)

免疫原

Human thymocytes and T lymphocytes

亚型

IgG2a
anti-CD4 (CD4) (AA 385-457) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 犬, Pig, 兔, Cat, Cow, 绵羊, 豚鼠, 山羊, 马, Avian, 驴, Hamster WB, IHC 宿主: 山羊 Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) (AA 300-400) antibody
CD4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 适用: 人 FACS, WB, MS 宿主: 小鼠 Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) (AA 26-200) antibody
CD4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 适用: 小鼠 FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE

应用备注

Immunoprecipitation. Functional Application: The antibody blocks binding of HIV gp120 to CD4 Molecule and it also strongly inhibits CD4-MHC Class II interactions. Flow Cytometry: Although it has not been tested rigorously, following data suggest that the antibody is a low-affinity antibody: its binding to T cells increases at elevated temperature, monovalent Fab fragments essentially do not bind to T cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

有效期

12 months
抗原

CD4

别名

CD4

背景

CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin Intracellular ligands: p56Lck CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

基因ID

920

UniProt

P01730

途径

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

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